WGS of Leishmania mexiacana TERT_KU80_mut - SRA

SRX25680668: WGS of Leishmania mexiacana TERT_KU80_mut
1 OXFORD_NANOPORE (PromethION) run: 229,491 spots, 3.1G bases, 2.7Gb downloads

Design: Genomic DNA (gDNA) from 2 10^8 L. mexicana cells was isolated using the phenol/chloroform method. DNA integrity was confirmed via 1% agarose gel electrophoresis. Samples were purified with the Short Read Eliminator XS kit (PacBio) and quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific). Approximately 1 g purified gDNA was used for nanopore sequencing library construction with the Native Barcoding Kit 24 V14 SQK-NBD114.24 (Oxford Nanopore Technologies) and sequenced on a PromethION Flow Cell.

Submitted by: Central European Institute of Technology, Masaryk University

Study: Ablation of LmKu80 activates alternative lengthening of telomeres in Leishmania mexicana

PRJNA1147409 • SRP525931 • All experiments • All runs

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The study investigates the impact on telomere maintenance in L. mexicana through knockout mutants of Ku80 and TERT, as well as a double-knockout of both genes. To provide insights into the behavior of telomeres we employed long-read nanopore sequencing for the analysis of chromosome arm-specific telomere reads.

Sample: TERT_KU80_mut

SAMN43153225 • SRS22322297 • All experiments • All runs

Organism: Leishmania mexicana

Library:

Name: TERT_KU80_mut

Instrument: PromethION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 229,491 spots, 3.1G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR30217729	229,491	3.1G	2.7Gb	2024-10-01

ID:: 34702963

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